FIG 5.

DksA positively regulates the transcription of SPI-1 and flagellar genes. (A) Total RNA was harvested from S. Typhimurium SL1344 and its isogenic dksA mutant strain cultures grown to mid-logarithmic phase at 37°C and was subjected to qRT-PCR. The change in the abundance of the indicated transcripts (normalized to rpoD) in the dksA mutant strain relative to wild-type S. Typhimurium is shown. Data are means and SEM from 3 to 6 independent RT-PCRs. (B) SDS-PAGE Western blot analysis of bacterial cell lysate from S. Typhimurium and dksA mutant strains expressing SopB-2HA, SopE2-2HA, and FliC-2HA grown to the mid-logarithmic phase. Protein fractions were probed using anti-HA antibody and anti-RpoD as a control.