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S. Typhimurium dksA is induced at the mid-exponential to late exponential phase in vitro. S. Typhimurium SL1344 harboring the regulatory regions of dksA and rpoD cloned into pCS26 (pCS26::PdksA and pCS26::PrpoD, respectively) were grown in LB broth for 24 h. At the indicated time points, optical density (OD600) and the activity of the promoters, presented in relative luminescence units (RLU), were determined. The means and SEM for three independent cultures are shown for each time point.
