FIG 7.
dksA is expressed at the midcecum during intestinal colonization in vivo. (A and B) Streptomycin-pretreated C57BL/6 mice were infected with 5 to 8 × 106 CFU of S. Typhimurium harboring pCS26 (A) or pCS26::PdksA (B). At 24 h p.i., the intestinal tracts and systemic sites (liver and spleen) were removed, and bioluminescence was imaged using a photon-counting system. Organs from two mice are shown from each infection. (C and D) To determine the total numbers of colonizing Salmonella (CFU), organs were homogenized in saline, diluted, and spread plated on XLD agar supplemented with kanamycin. Data are mean bacterial loads and SEM for three mice infected with S. Typhimurium carrying pCS26 (C) or pCS26::PdksA (D).