FIG 2.

IFN-γ responses to J-PPD in blood and fecal shedding of M. avium subsp. paratuberculosis in cattle experimentally infected with M. avium subsp. paratuberculosis. The dates of sample collection are indicated on the horizontal axis. The left vertical axis (gray circle) indicates the M. avium subsp. paratuberculosis (Map) DNA quantity in fecal samples determined using IS900 real-time PCR. The detection limit of this assay was 0.0001 pg/2.5 μl (19). Thus, the sample in which Map DNA was not detected is shown as “<0.0001 pg” on the graph. The right vertical axis (black diamond) shows IFN-γ production in whole blood cultures stimulated with J-PPD.