FIG 3.

Expression levels of PD-1 and LAG-3 on T cells in M. avium subsp. paratuberculosis-infected cattle. (a) Gating strategy and representative dot plots for expression analyses of PD-1 and LAG-3 on IgM⁻ CD3⁺ CD4⁺ and IgM⁻ CD3⁺ CD8⁺ T cells from the blood of M. avium subsp. paratuberculosis-infected cattle. Values in the quadrant indicate the percentages of cells. (b and c) Percentages of PD-1-expressing (b) and LAG-3-expressing (c) cells in IgM⁻ CD3⁺ CD4⁺ and IgM⁻ CD3⁺ CD8⁺ T cells of peripheral blood, spleen, ileocecal LN, ileal and jejunal mLNs, and ileal and jejunal PPs isolated from uninfected cattle (n = 3) and M. avium subsp. paratuberculosis-infected cattle (n = 7). Statistical comparisons between groups were performed using Welch's t test. Differences were considered significant when P < 0.05 (*, P < 0.05; **, P < 0.01). See also Table S1 in the supplemental material for individual expression data.