TABLE 1.

Primary antibodies used in flow cytometric analysis of this study

Target	Isotype	Clone	Company (reference)	Fluorochromea	Conjugation or labeling
Flow cytometric analysis of PD-1 and LAG-3
CD4	Mouse IgG2a	CC8	AbD Serotec	FITC	Conjugated primary antibody product
CD8	Mouse IgG2a	CC63	AbD Serotec	PerCp-Cy5.5	Lightning-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences)
CD3	Mouse IgG1	MM1A	VMRD	PE	Zenon R-PE mouse IgG1 labeling kit (Life Technologies)
IgM	Mouse IgG1	IL-A30	AbD Serotec	PE-Cy7	Lightning-Link PE/Cy7 conjugation kit (Innova Biosciences)
PD-1	Rat IgG2a	5D2	In house (13)	APC	APC-conjugated anti-rat immunoglobulin antibody (Beckman Coulter)
LAG-3	Rat IgG1	71-2D8	In house (this study)	APC	APC-conjugated anti-rat immunoglobulin antibody (Beckman Coulter)
Flow cytometric analysis of PD-L1 and MHC-II
CD14	Mouse IgG1	CAM36A	VMRD	APC-Cy7	Lightning-Link APC/Cy7 conjugation kit (Innova Biosciences)
CD11b	Mouse IgG2b	CC126	AbD Serotec	FITC	FITC-conjugated anti-mouse IgG2b antibody (Beckman Coulter)
MHC-II	Mouse IgG1	CAT82A	VMRD	PE	Zenon R-PE mouse IgG1 labeling kit (Life Technologies)
PD-L1	Rat IgG2a	4G12	In house (20)	APC	APC-conjugated anti-rat immunoglobulin antibody (Beckman Coulter)
Flow cytometric analysis of IFN-γ-producing T cells (intracellular staining)
CD4	Mouse IgG2a	CC8	AbD Serotec	FITC	Conjugated primary antibody product
CD8	Mouse IgG2a	CC63	AbD Serotec	PerCp-Cy5.5	Lightning-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences)
CD69	Mouse IgG1	KTSN7A	Kingfisher Biotech	Alexa Fluor 647	Zenon Alexa Fluor 647 mouse IgG1 labeling kit (Life Technologies)
IFN-γ	Mouse IgG1	CC302	AbD Serotec	PE	Conjugated primary antibody product

^aAPC, allophycocyanin; Cy, cyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCp, peridinin-chlorophyll-protein complex.