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. 2015 Mar 31;227(6):767–780. doi: 10.1111/joa.12283

Figure 2.

Figure 2

qRT‐PCR analysis investigating the expression of the endothelial markers (A) Angiopoietin 1, (B) VEGFR2, (C) CD31 and (D) vWF by AFSCs at day 7 of culture. AFSCs in growth media were used as a negative control and HUVECs were used as a positive control. AFSCs in all O2 conditions adopted an early stage endothelial gene expression profile. Significant increases in VEGFR2 and CD31 in continuous hypoxia indicated that continuous hypoxia enhanced the endothelial gene expression profile of AFSCs relative to AFSCs in growth media and the other O2 conditions. Endothelial gene expression in AFSCs in all three O2 conditions was significantly (P < 0.001) lower than the baseline expression of HUVECs. Values are expressed as mean ± SD, n = 4. ** P < 0.01 statistical significance differences relative to all other groups excluding HUVECs. *** P < 0.001 statistical significance differences relative to all other groups. # P < 0.001 statistical significance differences relative to HUVECs. ### P < 0.001 statistical significance differences relative to all other groups excluding HUVECs.