Figure 4.

VEGF secretion by AFSCs over the course of 14 days in response to endothelial stimuli in various O₂ conditions. VEGF secretion was significantly higher (P < 0.001) in continuous hypoxia at day 7 and 14. VEGF secretion in intermittent hypoxia was significantly (P < 0.05) higher relative to AFSCs in growth media and normoxia at day 7. AFSCs in growth media served as the negative control. HUVECs served as the positive control. Values are expressed as mean ± SD, n = 4. * P < 0.05 statistical significance differences relative to AFSCs in growth media and the normoxia group at day 7. *** P < 0.001 statistical significance differences relative to all other groups excluding continuous hypoxia at day 14.