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. 2015 Sep 15;4(6):879–895. doi: 10.1002/mbo3.286

Figure 2.

Figure 2

Strains lacking SltB1 and MltB die more slowly than wild‐type cells when treated with piperacillin. LT mutant cells were treated with piperacillin and their viability over 24 h was compared to untreated cells. Loss of sltB1/mltB slows piperacillin‐induced death over the first 3 h (A) with the remaining cells regrowing over 24 h (B). The growth rates between PAO1 and sltB1/mltB are the same (C), ruling out growth‐related differences in MIC. Asterisks indicate statistical differences between sltB1/mltB and PAO1. N = 3. Bars represent the mean ± SEM. *P < 0.05; **P < 0.01. LT, lytic transglycosylase; MIC, minimum inhibitory concentration.