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Combination mutants lacking mLTs or Family 1 LTs are hyperbiofilm formers. Biofilms of LT mutants grown for 24 h were compared to those formed by wild type. (A) Loss of multiple mLTs increased biofilm formation while (B) the loss of sLTs did not. N = 3. Bars represent the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. mLT, membrane‐bound lytic transglycosylase; sLT, soluble lytic transglycosylase.
