Table 2.
Correlation between cytoplasmic GFP signal patterns and mCherry leakage in the compatible interaction
| GFP signals | Test | mCherry signals | ||
|---|---|---|---|---|
| Normal infection loci | Abnormal infection loci | Ratio (mCherry/GFP) | ||
| Normal | 1 | 1282 | 0 | |
| 2 | 1218 | 1 | ||
| 3 | 912 | 0 | ||
| Total | 3412 | 1 | 0.03%a | |
| Abnormal | 1 | 8 | 45 | |
| 2 | 7 | 17 | ||
| 3 | 7 | 36 | ||
| Total | 22 | 98 | 81.7%b | |
| Ratio (GFP/mCherry) | 0.60%c | 99.0%d | ||
In each test, the sixth leaf sheath of five transgenic rice plants expressing AcGFP1 (cyto‐GFP line) were inoculated with a virulent strain expressing mCherry (TmC line) and the fluorescence patterns were evaluated for each appressorium (infection locus, 3533 loci in total) by microscopy at 30 hpi. GFP, green fluorescent protein.
a
Abnormal mCherry/normal GFP.
b
Abnormal mCherry/abnormal GFP.
c
Abnormal GFP/normal mCherry.
d
Abnormal GFP/abnormal mCherry.