Table 2.

Primary and secondary antisera used in the study

Antigen	Host	Dilution	Supplier	Catalogue number
Primary
ChAT	Goat	1 : 100	Chemicona	AB144P
TH	Mouse	1 : 400	Chemicona	MAB318
TH	Sheep	1 : 800	Chemicona	AB1542
CGRP	Mouse	1 : 800	Abcamb	AB5920
SP	Guinea pig	1 : 1000	Abcamb	AB1053
PGP 9.5	Mouse	1 : 200	AbD Serotecc	7863‐1004
NOS1	Mouse	1 : 500	Santa Cruz Biotechonologyd	SC‐5302
Secondary
GoatCy3	Donkey	1 : 300	Chemicona	AP180C
MouseCy3	Donkey	1 : 300	Chemicona	AP192C
MouseDyLight ®488	Donkey	1 : 300	Abcamb	AB96875
MouseFITC	Donkey	1 : 100	JacksonImunoresearche	715‐095‐151
SheepFITC	Donkey	1 : 100	Chemicona	AP184F
Guinea pigFITC	Donkey	1 : 100	Chemicona	AP193F

^aChemicon International, Temecula, California, USA.

^bAbcam, Cambridge, UK.

^cAbD Serotec, Kidlington, UK.

^dSanta Cruz Biotechonology, Dallas, Texas, USA.

^eJackson ImmunoResearch, West Grove, Pennsylvania, USA.

CGRP, calcitonin gene‐related peptide; ChAT, choline acetyltransferase; NOS, nitric oxide synthase; SP, substance P; TH, tyrosine hydroxylase.