Figure 1.

IR‐based tissue section scanning yields images of suitable quality for accurate morphometric measurements. H&E tissue sections can be imaged on an IR scanner. (A) Photographic representation of approximate murine tissue section levels. Representative sections at levels 1–6 are shown in (B) and (C). (B, C) Frozen 20‐μm‐thick tissue H&E‐processed sections were scanned with a Hamamatsu Nanozoomer‐ XR slide scanner (B) and LI‐COR Odyssey imager at 700 nm (C). Areas of anatomical interest within each section, such as: (1) whisker pouches in the nose; (2) emergence of the eyes and olfactory cavity; (3) eye sockets and thalamus; (4) hippocampus; (5) midbrain with cerebellum; and (6) spinal cord. (D) Measurements from each section were obtained using either imagej software applied to Hamamatsu nanozoomer‐XR images or imagestudio lite software on the LI‐COR IR scanner. Scale bars (black for B and white for C): 1 mm.