Figure 3.

Knockdown β-catenin reduced the promotion of FOXH1 in cell proliferation and invasion. A. RT-qRCR for β-catenin and cyclinD1 mRNA and Immunoblotting for the relative protein using lysate from MCF-7 cells stably transfected with a vector or FOXH1-transfected cells. B. The above experiments were performed in MDA-MB-231 cells stably transfected with a vector or FOXH1-transfected cells. C. Cell proliferation assay and transwell assay were performed in FOXH1 stable transfected MCF-7 cells, follow by transiently transfected with a control siRNA or a β-catenin targeted siRNA and treated for 24 hours. D. The similar experiments were performed in FOXH1 stable transfected MDA-MB-231 cells, follow by transiently transfected with a control siRNA or a β-catenin targeted siRNA and treated for 24 hours.