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. 2015 Oct 15;8(10):17601–17611.

Figure 4.

Figure 4

BITC treatment has remarkable effect on transcriptional activity of FOXH1. A. qRT-PCR and western blot analysis for FOXH1, β-catenin and GAPDH mRNA levels and protein levels in MCF-7 cells treated with DMSO (control) or the indicated concentrations of BITC for 16-24 h. Results shown are mean ± SD (n = 3). *Significantly different (P<0.05). B. qRT-PCR and western blot analysis for FOXH1, β-catenin and GAPDH mRNA levels in MDA-MB-231 cells. C. qRT-PCR and western blot analysis for FOXH1, β-catenin and GAPDH mRNA levels and protein levels in SUM159 cells. D. β-catenin luciferase reporter activity (as measurement of FOXH1 transcriptional activity) in MCF-7, MDA-MB-231, and SUM159 cells after treated with DMSO or the indicated concentrations of BITC for 24 h Each experiment was repeated at least three times. Results were shown as mean ± SD. *Significantly different (P<0.05).