Figure 5.

ISO suppressed NLRP3 inflammasome activation in the kidney of MRL/lpr mice. A. Western blot was performed to analyze the expression of NLRP3, ASC, and caspase-1 p20. b-actin was used as the endogenous control. B. Quantitative expression of NLRP3, ASC, and caspase-1 p20 was normalized against b-actin. C and D. Renal and serum IL-1β levels were measured by ELISA. Each experiment was performed in triplicate. Values are presented as mean ± SD. **P < 0.01 vs. Ctrl groups; #P < 0.05, ##P < 0.01 vs. MRL + RA group. Ctrl: control; RA: room air; ISO: isoflurane.