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. 2015 Oct 15;8(10):18765–18771.

Figure 1.

Figure 1

RASAL2 was down-regulated and inhibited proliferation in nasopharyngeal carcinoma. A. The relatively decreased level of RASAL2 was detected via real-time PCR in 22 nasopharyngeal carcinoma tissues compared with 8 normal tissues. B. The mRNA expression level of RASAL2 in CNE-1 cells was highest compared with SUNE-1, CNE-2, and HNE-2 cells. C. The mRNA expression level of RASAL2 in CNE-1 cells with siRNA treated by real-time PCR assay. The mRNA expression level of GAPDH was served as control. D. Western blotting assays showed decreased expression of RASAL2 in CNE-1 cells with siRNA treatment compared with scramble sequence treatment. The quantification of experiments was shown in right panel. E. The CCK-8 assay showed that a decreased level of RASAL2 promoted the growth of CNE-1 cells. Absorbance at 450 nm was presented as the mean ± SEM. All experiments were performed in triplicate and presented as the mean ± SEM. *, indicates a significant difference compared with the control group (P<0.05).