Table 3.
Hydrogen production after anaerobic growth in rich media
| straina |
genotype |
hydrogenb |
OD600nm |
|---|---|---|---|
| nmol OD600−1 ml−1 | |||
| MC4100 | – | 5394 ± 190 | 1.71 |
| FTD147 | ΔhyaB, ΔhybC, ΔhycE | <1 | 1.87 |
| Teatotal1 | ΔhyaB, ΔhybC, ΔhycE, ΔadhE | <1 | 1.00 |
| FTD147h3 | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD | <1 | 0.40 |
| FTD147h3 + plasmidsc | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD | <1 | 0.59 |
| CPD159F | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, pflA | <1 | 1.70 |
| CPD159F + plasmidsc | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, pflA | <1 | 1.53 |
| CLK001 | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, ΔiscR | <1 | 0.63 |
| CLK001 + plasmidsc | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, ΔiscR | 28.7 ± 5.1 | 0.48 |
| CPD152E | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, ΔiscR, ΔpflA | <1 | 1.59 |
| CPD152E + plasmidsc | ΔhyaB, ΔhybC, ΔhycE, ΔadhE::hydABCD, ΔiscR, ΔpflA | 8.4 ± 3.3 | 1.35 |
The mean and standard deviation of at least three independent measurements are shown.
a
Cultures were grown anaerobically in TGYEP, pH 6.5 in closed Hungate tubes at 37 °C for 17 h.
b
H2 content was determined from headspace gas phase by gas chromatography.
c
denotes addition of accessory genes (pSUtat-Sh-GX-EF) and a plasmid encoding Th. maritima POR and ferredoxin (pUNI-Tm-POR-Fd).