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. Author manuscript; available in PMC: 2015 Dec 29.
Published in final edited form as: Nat Neurosci. 2015 Oct 5;18(11):1584–1593. doi: 10.1038/nn.4132

Figure 4.

Figure 4

Reduced population spike responses in AAV-GFP/tau mice are rescued by depletion of microglia. (a) Field potential responses in the dentate granule cell layer to a 100-μA stimulus in the middle molecular layer in hippocampal slices prepared from the following: left, AAV-GFP (control); middle, AAV-GFP/tau; right PLX3397-treated (microglia depleted) AAV-GFP/tau mice. Vertical black line represents time of stimulus and asterisk denotes the population spike. (b) Mean input-output relationships for the excitatory postsynaptic potential (EPSP) slope for all fields in slices prepared from AAV-GFP mice fed control chow (open circles; n = 3 mice, 16 slices), AAV-GFP/tau mice fed control chow (filled circles, n = 3 mice, 10 slices), AAV-GFP mice fed PLX3397 chow (open triangles; n = 3 mice, 16 slices) and AAV-GFP/tau mice fed PLX3397 chow (filled triangles, n = 3 mice, 19 slices). (c) Mean input-output relationships for the population spike for all fields in the same groups as b. Error bars in b and c represent s.e.m. P = 0.0003, F(14,364) = 2.932 for AAV-GFP + control versus AAV-GFP/tau + control; P = 0.0605, F(14,350) = 1.668 for AAV-GFP/tau + control versus AAV-GFP + PLX3397; P = 0.0057, F(14,392) = 2.263 for AAV-GFP/tau + control versus AAV-GFP/tau + PLX3397, as determined by two-way repeated-measurement ANOVA.