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. Author manuscript; available in PMC: 2015 Dec 29.
Published in final edited form as: Nat Neurosci. 2015 Oct 5;18(11):1584–1593. doi: 10.1038/nn.4132

Figure 7.

Figure 7

nSMase2 silencing or inhibition reduces exosomal secretion and exosome-mediated tau transmission from microglia to neurons. (a,b) Primary cultured microglia were either transfected with siRNA against murine nSMase2 gene or scrambled siRNA (a) or treated with 3 μM GW4869 in 0.5% DMSO in DMEM for 24 h (b), followed by treatment with oligomeric hTau, LPS and ATP as described for Figure 5a. The exosomal fraction (EF) from microglia (10 μg per lane) was immunoblotted with Tau46 and Tsg101. (c,d) Microglia-derived exosome-treated neuronal cell lysates were immunoblotted for hTau (HT7) or β-actin. Results are representative of 3 independent experiments. Full-length blots are presented in Supplementary Figure 10.

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