Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 30;98(1):175–192. doi: 10.1111/mmi.13114

Figure 4. CDI expression is required for auto-aggregation.

Figure 4

A) CH9591 (bamAECL) cells were transformed with pTNC-WEB (CDI−) or pDAL660Δ1-39 (CDI+) plasmids and labeled with GFP or DsRed. Each of the four cell populations was analyzed by flow cytometry to detect background levels of dual green/red fluorescent events. B) The individual cell populations show in panel A were mixed at 1:1 ratio and incubated with shaking at 37 °C. The suspensions were then analyzed by flow cytometry using FL1 (533/30nm, GFP) and FL2 (585/40nm, DsRed) fluorophore filters.