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. 2015 Dec 29;9(12):e0004279. doi: 10.1371/journal.pntd.0004279

Fig 4. CYP450 messenger RNA abundance during the lifecycle of Schistosoma mansoni.

Fig 4

Whole RNA was extracted from different stages of S. mansoni (cercariae, 1-day old schistosomula; juvenile liver worms (23 days post infection), adult males (49 days post infection), adult females (49 days post infection) and eggs) using TRIzol reagent and chloroform/ethanol extraction protocol. cDNA was synthesized from whole RNA and used for qRT-PCR, with reactions done in triplicate. Adult males (= 1) were used as calibrator stage and mRNA abundance was normalized to α-tubulin. Error bars indicate standard error of the mean with n ≥ 3 biological replicates. Numbers indicate fold change relative to adult males and all values are significantly different from adult males; p < 0.05; student t-test. The results indicate that S. mansoni CYP450 is expressed in all stages investigated and that its expression is developmentally regulated.