Table 2. List of primers used for PCR, RT-PCR and qRT-PCR.
Primer | Sequence |
---|---|
T3 | GCTCGAAATTAACCCTCACTAAAGGG |
SK | CGCTCTAGAACTAGTGGATC |
SL | AACCGTCACGGTTTTACTCTTGTGATTTGTTGCATG |
P450_5' | ATGGATACCTTTGAATTTTATG |
P450_3' | TTACTTCCATACATCGGTACG |
CYP450-R1 | CACCATAAGTTGCAACAACG |
CYP450-R3 | GTTGAGAAGCAGACACATCC |
CYP450-revComF2 | CTGACTATTGTGTACAGCATA |
SmCYP450-For | GTGGACAATTCTGTTGTCTA |
SmCYP450-Rev | CTCCAAACTGTACATCTCATC |
T7-T3 | TAATACGACTCACTATAGGGATTAACCCTCACTAAAGGGA |
qPCR-p450-F | TGCTGGTACTGACACCACGTCTTT |
qPCR-p450-R | GGAACTACACTAGCCCAACGATGA |
qPCR-tubulin-F | CACGAGCAGTTAAGCGTTGCAGAA |
qPCR-tubulin-R | TATTTGCCGTGACGAGGGTCACAT |
CYP450-interF2 | TATGCTGTACACAATAGTCAG |
ORF_CYP450 Reverse | TTAAATACTTGTTCTTCTATTTCC |
GAPDH_S.mansoni FWD | ATGTTCGTTGTTGGTGTGAATG |
GAPDH_S.mansoni REV | TTCCGTTTATGTCTGGAATGA |