Fig 3. Effect of butyrate on adiponectin protein expression and secretion.

(A) Representative immunoblotting and quantification analysis of adiponectin abundance in culture media. (B) Representative immunoblotting analysis of intracellular adiponectin level in the cell lysate. Preadipocytes were treated with butyrate at 1500 μM concentration throughout the differentiation period (day 0 to 9). Both cells and culture media were harvested on day 9 for immunoblotting. Data were analyzed by t test, and P values were shown on the bars (Control vs. Butyrate). Data are means ± SE (n = 4).