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. 2015 Dec 29;10(12):e0145940. doi: 10.1371/journal.pone.0145940

Fig 12. Interaction between butyrate and fatty acid oxidation inhibitors on PPARγ abundance.

Fig 12

(A) Representative immunoblotting analysis of PPARγ in adipocytes. (B) Quantification analysis of PPARγ in adipocytes. Cells were treated with etomoxir (ETO) at 1 μM, thioridazine (TRD) at 10 μM or a combination of the two (TE). Inhibitors were applied with/without butyrate (But) at 1500 μM concentration throughout the differentiation period (day 0 to 9). Cells were harvested at day 9 for immunoblotting. Data were analyzed by two-way ANOVA with Tukey multiple comparison test. Data are means ± SE (n = 8). Different letters on bars indicate significant differences (P<0.05).