Figure 2.
Lactate release by hepatoma cell lines was reduced following treatment with 5 nm AgNPs for 1 hour or more.
Notes: HepG2 (3×105/mL, A, C, and E) and Huh7 cells (2×105/mL, B, D, and F) were treated with 5 µg/mL 5 or 100 nm AgNPs for the indicated times. Glucose consumption (A and B) and lactate release (C and D) in culture media were measured using a YSI biochemical analyzer. Cell viability (E and F) was analyzed by labeling with propidium iodide and flow cytometry. The data represent the mean ± standard deviation of two independent experiments conducted in hextuplicate. *P<0.005.
Abbreviation: AgNPs, silver nanoparticles.