Table 4. Embryonic lethality of endothelial-specific (EC)-Rap1 KO mice.
| Genotype | Total embryo number | Expected */ | Abnormal | Viable | Viable/Expected | Comments |
|---|---|---|---|---|---|---|
| Tie2-Cre+/0 Rap1af/f Rap1bf/f | 2 | 4.75 | 2 | 0 | 0% | Smaller size, hemorrhage at base of skull |
| Tie2-Cre0/0 Rap1af/f Rap1bf/f | 7 | 4.75 | 1 | 6 | ≥100% | |
| Tie2-Cre+/0 Rap1af/f Rap1bf/+ | 3 | 4.75 | 1 | 2 | 42% | Hemorrhage, blood clots, edema |
| Tie2-Cre0/0 Rap1af/f Rap1bf/+ | 5 | 4.75 | 0 | 5 | ≥100% | |
| Tie2-Cre+/0 Rap1af/+ Rap1bf/f | 7 | 4.75 | 2 | 5 | ≥100% | |
| Tie2-Cre0/0 Rap1af/+ Rap1bf/f | 7 | 4.75 | 1 | 6 | ≥100% | |
| Tie2-Cre+/0 Rap1af/+ Rap1bf/+ | 5 | 4.75 | 0 | 5 | ≥100% | |
| Tie2-Cre0/0 Rap1af/+ Rap1bf/+ | 4 | 4.75 | 0 | 4 | 84% |
Number of E15.5 embryos obtained from Rap1af/f Rap1bf/f x Tie2 Cre+/0; Rap1af/+ Rap1b f/+ crosses, per genotype.
Total number of embryos = 38.
*/ Expected number = Total number * 1/8 frequency