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. 2016 Jan 1;27(1):137–152. doi: 10.1091/mbc.E15-09-0650

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© 2016 Tang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Actin filaments are required for Golgi integrity. (A) HeLa cells treated with DMSO, cytochalasin B (CytB, 40 μM), or latrunculin B (LatB, 0.5 μM) for 2 h were stained for α-mannosidase II (ManII) and rhodamine-labeled phalloidin. (B) Cells transfected with indicated siRNA were stained for ManII and rhodamine–phalloidin. Bar, 20 μm (A, B). (C) Cells treated with DMSO, cytochalasin B, or latrunculin B for 2 h were processed for EM and imaged. Arrows indicate Golgi stacks. The average cisternal length of Golgi stacks was quantified. Statistical significance was assessed by comparison to DMSO-treated cells. (D) Cells transfected with control, β-actin, or γ-actin siRNA for 48 h were processed for EM and analyzed as in C. Statistical significance was assessed by comparison to the control siRNA-treated cells. Bar, 500 nm (C, D). ***p < 0.001.