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. 2016 Jan 1;27(1):137–152. doi: 10.1091/mbc.E15-09-0650

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© 2016 Tang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Mena and F-actins enhance GRASP65 oligomerization. (A, B) GRASP65-coated beads were incubated with interphase cytosol (IC) immunodepleted by nonspecific or anti-Mena IgG. Beads were imaged by microscopy (A), and the percentage of beads in aggregates was quantified (B). (C, D) GRASP65-coated beads were incubated with IC in the presence of DMSO or 40 μM cytochalasin B and results quantified. (E, F) GRASP65-coated beads incubated with indicated purified proteins or IC were imaged and quantified. (G, H) GRASP65-coated beads incubated with interphase cytosol or interphase cytosol that was preincubated with BSA, Mena EVH1, domain or Mena EVH2 domain were imaged and quantified. Bars, 50 μm. ***p < 0.001.