FIGURE 1:

LMNB1 overexpression reduces axon outgrowth in mouse cortical neurons. Primary cortical neurons were transfected before plating by nucleofection with pLMNB1-EGFP or pEGFP. Axonal and dendritic outgrowth was analyzed 7 d later as described in Materials and Methods. (A, B) Representative immunofluorescence images of Tau immunoreactivity (red) in 7 DIV neurons transfected with pEGFP (A) and pLMNB1-EGFP (B). Nuclei are counterstained with DAPI (blue). Scale bars, 20 μm. (C, D) Quantitative analysis of axonal (C) and total dendritic (D) length. Bars represent the mean length ± SEM of 65 neurons/group measured in three independent experiments. *p < 0.05, Student’s t test. (E) Sholl analysis of dendritic tree arborization.