FIGURE 4:

Timing of DNA replication relative to HOC across strains. Strains (A) CEN.PK, (B) DBY12007, (C) YJM128, and (D) YPS670 were cultured in chemostat at the same dilution rate (D = 0.1 h⁻¹). We extracted samples every 10 min and measured CDC events (DNA content) and YMC events (pO₂); see Materials and Methods. For each strain, the raw DNA content, pO₂, S/G₂/M fraction, and S fraction are plotted over several YMCs (left). The average over each YMC is plotted to the right of each full data set, where t = 0 corresponds to entry into HOC. We plot the 50% midpoint of S/G₂/M fraction (solid red circle) and 100% peak of S fraction (solid green circle). The time of DNA replication (50% of S/G₂/M fraction) after entry into HOC is defined as Δ, whereas time of DNA replication (100% of S fraction) is Δ_S.