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. 2016 Jan 1;27(1):75–89. doi: 10.1091/mbc.E15-05-0319

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© 2016 Sun et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — GST-MICAL-L2-ACT binds Rab13, ACTN4, and GLUT4. (A) Lysates from L6 GLUT4myc cells expressing GFP-Rab13 or GFP, stimulated with insulin or not, were pulled down with GST-MICAL-L2-ACT (containing Rab- and ACTN4-binding domains) and immunoblotted with anti-GFP and anti-ACTN4. Phospho-Akt illustrates insulin effectiveness. Pull down by GST-MICAL-L2-ACT of (B) GFP-Rab13, (C) ACTN4, and (D) GLUT4myc (n = 6, mean ± SE, *p < 0.05; ns, not significant).