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. 2016 Jan 1;27(1):75–89. doi: 10.1091/mbc.E15-05-0319

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© 2016 Sun et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Insulin-elicited three-way colocalization of MICAL-L2, ACTN4, Rab13, and GLUT4 at cellular ruffles. (A) L6 cells cotransfected with GFP-MICAL-L2 and MC-Rab13 were stimulated with insulin or not and analyzed by spinning-disk confocal fluorescence microscopy. Collapsed optical z-stack images of GFP-MICAL-L2 (green), MC-Rab13 (red), and ACTN4 (blue). (B) L6 cells cotransfected with GFP-Rab13 and MC-GLUT4 and labeled for ACTN4. Collapsed optical z-stack images of GFP-Rab13 (green), MC-GLUT4myc (red), and ACTN4 (blue). All results are representative of three experiments (>25 cells/condition). Scale bars, 10 μm.