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. 2015 Sep 5;6(28):24611–24626. doi: 10.18632/oncotarget.5232

Figure 1. Phenotypic characterization of the experimental model.

Figure 1

A. FACS analysis of splenic cells after sorting for the B220 isoform of CD45. B cells (B220+ cells) are analyzed for B220 and IgM expression and non-B cells (B220− cells) for B220 and CD3ε expression. The data represent the average ± standard deviation of flow cytometric analyses of twelve mice. B. p53 stabilization is induced by DNA damage in B and non-B cells from Trp53+/+ mice treated with 7 Gy of irradiation (IR) compared to control animals (mock) and Trp53−/− animals. Four hours after IR, spleens are harvested from each mouse and B and non-B cells are purified by cell sorting for B220. Protein lysates from individual mouse cells are subjected to western blot analysis with antibodies specific for the indicated proteins. Phosphorylation of H2AX is shown as a positive control for DNA damage. C. The accumulation of cells with sub-G1 DNA content is examined using flow cytometry in B and non-B cells from control (mock) and irradiated (IR) Trp53+/+ and Trp53−/− mice. The reported values represent the average ± standard deviation of three biological replicates. *p < 0.05 (Student's t-test) D. Representative flow cytometric analysis of DNA content (Propidium iodide staining) of B and non-B cells from control (mock) and irradiated (IR) Trp53+/+ and Trp53−/− mice.