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. 2015 May 29;6(28):24636–24648. doi: 10.18632/oncotarget.4323

Figure 1. Zinc Finger Nucleases Knock out of BASIGIN/CD147 gene in tumour cells.

Figure 1

A. Schematic representation of the four isoforms of human BASIGIN (BSG-1, BSG-2, BSG-3 and BSG-4), and mapping of the human BASIGIN gene sequence (exons/introns) with the four mRNA sequences for human BASIGIN spliced isoforms adapted from [29]. Two ZFN were used to generate BSG−/− cell lines; ZFN1 targeted the exon 2 encoding peptide signal and common to BSG-1 and BSG-2, while ZFN2 was designed to target the exon 7, encoding a part of the transmembrane domain and common to all the isoforms. B. Western blot analysis. LS174T, U87 and A549 wt cells and BSG−/− clones were grown in normoxia during 48h and lysed. BASIGIN expression was analysed by immunoblotting. ARD1 was used as loading control. C. Zymogel analysis of Conditioned Media (CM) from wt vs BSG−/− tumour cells lines. Cells were grown for 48h in 2% FBS media (lane a). Conditioned media were harvested, and MMP2/MMP9 activities were analysed by loading ECM samples in a 10% polyacrylamide zymogel.