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. 2015 Jul 28;6(28):24842–24855. doi: 10.18632/oncotarget.4704

Figure 2. Micromechanical properties of immunopurified MUC16/PODXL binding to E/L-selectin using single molecule force spectroscopy.

Figure 2

Rupture force as a function of loading rate for immunopurified A. MUC16-E/L-selectin and B. PODXL-E/L-selectin interaction. Data represent the mean ± S.E.M. of 3–4 experiments run for each binding pair. Least-squares fitting of the Bell model were used to determine kinetic parameters. C. Rupture force distribution was obtained both experimentally (bars) and computationally through Monte-Carlo simulations (line) based on Bell model kinetic parameters for immunopurified PODXL-E-selectin binding. D-E. Frequency of binding between immunopurified MUC16 and PODXL with E-selectin and L-selectin in the absence and presence of the divalent cation chelator EDTA. Tips coated with 10 μg/ml E-/L-selectin were brought in contact with either MUC16 or PODXL incorporated lipid bilayers. The frequency of binding was measured with and without the presence of 10 mM EDTA. Data represent the mean ± S.E.M. of 3–4 independent experiments. *P < 0.05.