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. 2015 Jul 20;6(28):24884–24894. doi: 10.18632/oncotarget.4670

Figure 1. T cells are effectively transduced with GD2 CAR encoding vector.

Figure 1

A. The GD2 CAR construct. The IgM derived anti-GD2 scFv is linked to the signal transduction domain (STD). B. Replicate samples of anti-GD2 immunized mice sera (M1, M2, M3 and M4) efficiently recognize GD2 CAR on FLYRD18 cell surface and are introduced for GD2 CAR detection on transduced T cells. Isotype (gray), APC-secondary Ab (broken/gray line) and GD2 positivity (black line). C. GD2 CAR T cells were analysed for both GFP and CAR expression levels (48 ± 2% and 40 ± 10%, respectively, p > 0.05 by t-test) while GFP only T cells were exclusively GFP positive (64 ± 5%) Mean ± SEM. D. representative phase 1 (Ph1) and green fluorescence (GFP) photomicrographs (scale bar, 50 μm) of non-transduced (NT) pre-stimulated T cells (left panel) and GD2 CAR T cells after gene transfer revealing GFP-positive clusters in two representative donors (D) in middle (D1) and right (D2) panels.