Table 1b. Kinetic constants and binding affinities of anti-EpCAM mAbs.
| mAb colon | Kd(M) | Kon(M−1S−1) | Koff(S−1) |
|---|---|---|---|
| EpAb1-3 | 1.833 × 10−9 | 1.849 × 105 | 3.389 × 10−4 |
| EpAb2-6 | 3.491 × 10−10 | 4.007 × 105 | 1.399 × 10−4 |
| EpAb3-5 | ≤ 4.66 × 10−13 | 2.961 × 106 | 1.38 × 10−6 |
| EpAb4-1 | 1.228 × 10−12 | 2.865 × 105 | 3.519 × 10−7 |
| EpAb5-4 | 2.431 × 10−10 | 6.221 × 105 | 1.513 × 10−4 |
| hEpAb2-6 | 6.773 × 10−10 | 3.756 × 105 | 2.544 × 10−4 |
Kon and Koff were measured by SRP in a BIAcore using purified mAb, and the Kd was calculated by BIAevaluation software.