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. 2015 Jul 30;6(28):25295–25307. doi: 10.18632/oncotarget.4716

Figure 1. Microfluidic co-culture platform to study the interactions between carcinoma aggregates and macrophages.

Figure 1

A. Photograph of the polydimethyl siloxane (PDMS) device. B. Schematic images of an enlarged, isometric view of the channel layout showing the orientation of co-culturing carcinoma cell aggregates and endothelial cells (HUVECs), with macrophages either physically contacting (left panel; 1: media channel; 2: A549 aggregates and macrophages gel channel; 3. supporting gel channel; 4: HUVEC monolayer) or cultured under separated conditions (right panel; 1: media channel; 2: A549 aggregates gel channel; 3. macrophages gel channel; 4: HUVEC monolayer). C. HUVEC monolayers formed in the microfluidic channel. Green: GFP-HUVECs. D–F. E-cadherin immunocytochemical staining. E-cadherin expression of A549 aggregates at 0 h (D), E-cadherin expression of A549 aggregates in the absence (E) or presence (F) of macrophages at 36 h. Green: E-cadherin staining, red: mCherry A549 nuclei.