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. 2015 Jul 29;6(28):25320–25338. doi: 10.18632/oncotarget.4659

Figure 8. Essential role of TNFR1 in mediating the function of CTGF signaling.

Figure 8

A–B. A precedent treatment with anti-TNFR1 monoclonal antibody (10 μg/ml) more profoundly prevented the increase of CTGF-induced TNFR1 expression in the CTGF-highly expressed cells (over-CTGF-HMLER and HMLER-snail) than that in the CTGF-lowly expressed cells (control-HMLER and shCTGF2). C. Representative phase-contrast images showing the cell morphology changes in the indicated cell lines and treatments. D–E. The activation of IKBKA and IKBKB by CTGF could be specifically attenuated by the anti-TNFR1 antibody in the CTGF-high tumor cells. F. JUK but not ERK1/2 and p38 in the MAPK signaling pathway was up-regulated by exogenous CTGF in the CTGF-low tumor cells. G. Knockdown CTGF in the HMLER-snail cells down-regulated the IKBKA and IKBKB, and such down-regulation could be rescued by exogenous CTGF. All experimental data are representative of at least two independent experiments performed in triplicate. Data are expressed as mean ± s.e.m.