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. 2015 Jul 25;6(28):25484–25498. doi: 10.18632/oncotarget.4688

Figure 6. Knockdown of LIFr decreases STAT3 mRNA levels and phosphorylated STAT3 expression.

Figure 6

Forty-eight hours after transfection, cells were plated for migration assays, and the remaining cells were harvested for LIFr and STAT3 mRNA expression detection and Western blot analysis. a. Representative images of LIFr, phosphorylated STAT3 (p-STAT3) and b-actin expression in Western blotting. Analyses of band density are presented as the relative ratio of LIFr and STAT3 to actin for MMRU cells b. and PMWK cells c, d. LIFr mRNA expression in MMRU and PMWK cell lines after LIFr knockdown relative to controls (set to value of 1 fold). e. STAT3 mRNA expression in MMRU and PMWK cell lines after LIFr knockdown relative to controls (set to value of 1 fold). The data was analyzed by Student's t-test, Bars equal to mean ± SD. All experiments were carried out in triplicate. *P < 0.05, **P < 0.01.