Skip to main content
. 2015 Jul 23;6(28):25784–25800. doi: 10.18632/oncotarget.4703

Figure 4. Contribution of calcitriol and TGFβ to the AF4-MLL-dependent reporter gene activation by calcitriol/TGFβ.

Figure 4

HeLa cells were transfected with the pGL3cdsInJM reporter gene construct as well as the expression vectors for the nuclear receptors VDR and RXR and were co-transfected either with the pTarget control vector or the expression vectors for AF4 or AF4-MLL as indicated. 16 hours after transfection, cells were incubated without or with calcitriol (50 nM) and/or TGFβ (1 ng/ml). After 40 hours, luciferase activity was determined and results are given as x-fold induction over untreated cells. Each experiment was performed in triplicate. Results are presented as mean − S.E.M. of three independent experiments. Two-tailed t-test was performed to determine significant differences in relation to co-transfection of the backbone vector pTarget, indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).