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. 2015 Jul 23;6(28):25784–25800. doi: 10.18632/oncotarget.4703

Figure 5. Effect of the proteasome inhibitor MG132 on reporter gene activation by AF4 and AF4-MLL.

Figure 5

HeLa cells were transfected with the pGL3cdsInJM reporter gene construct and the expression vectors for the nuclear receptors VDR and RXR and were co-transfected either with the pTarget control vector or the expression vectors for AF4 or AF4-MLL. 16 hours after transfection, cells were incubated with calcitriol (50 nM) and TGFβ (1 ng/ml). Cells were treated with MG132 (5 μM) for 1, 6 and 16 hours. 40 hours after transfection, luciferase activity was determined and the results are given as RLU which include normalization to transfection efficiency. Each experiment was performed in triplicate. Results are presented as mean − S.E.M. of three independent experiments. Two-tailed t-test was performed to determine the significance in relation to MG132 untreated cells, indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).