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. 2015 Jul 23;6(28):25784–25800. doi: 10.18632/oncotarget.4703

Figure 7. Co-immunoprecipitation of AF4 and VDR/RXR.

Figure 7

HEK293T cells were co-transfected with the expression vectors for AF4 and the nuclear receptors VDR and/or RXR as indicated. Then, cells were cultured for 48 hours. After harvest and cell lysis, immunoprecipitation was performed with VDR or RXR antibodies as indicated. Subsequently, supernatants (I) and bead eluates (E) were analyzed by Western blot analysis for VDR, RXR and AF4. Figure illustrates one representative Co-IP of three independent experiments.