Skip to main content
. 2015 Jul 23;6(28):25784–25800. doi: 10.18632/oncotarget.4703

Figure 8. Effect of A. HDAC inhibitors and B. CDK9 inhibitor Flavopiridol on the AF4-MLL-dependent induction of reporter gene activity by calcitriol/TGFβ.

Figure 8

HeLa cells were transfected with the pGL3cdsInJM reporter gene construct plus the expression vectors for VDR and RXR (left panel) or the pN10 reporter gene construct (right panel). Additionally, cells were co-transfected either with the pTarget control vector or the expression vector for AF4-MLL as indicated. 16 hours after transfection, cells were incubated without or with calcitriol (50 nM) and TGFβ (1 ng/ml) and the indicated HDAC inhibitors or the CDK9 inhibitor Flavopiridol. After 40 hours, luciferase activity was determined. Results are given as RLU which include normalization to transfection efficiency. Each experiment was performed in triplicate. Results are presented as mean + S.E.M. of three independent experiments. Two-tailed t-test was performed to determine the significance of inhibitor treatment, indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001).