Figure 6. TRAIL-resistance of human mammary gland epithelial cells was due to malfunction of its recruitment to the cell surface membrane.

Top graphs; Breast cancer stem-like CSC-1 and the parent MCF10A cells were treated with rTRAIL (7, 10, 30, 50, 70, 100 ng/ml) for 24 h. The cell viability was estimated at 24 h after the treatment. Data were obtained from 3 independent experiments. The cell viability of the control (0; DMSO alone) is indicated as 100%. The growth inhibitory activity (IC₅₀) of each compound is indicated in each panel. Lower blot: Western blot analysis was performed to determine the steady-state expression of DR5, β-actin as the internal control. Lower photomicrographs: Oligomerization of DR5. Nuclei were counterstained in blue with Hoechst33342. Anti-DR5 bound to the cell surface is indicated by white arrows