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. 2015 Jul 16;6(28):25828–25842. doi: 10.18632/oncotarget.4558

Figure 9. Overexpression of DR5 induced TRAIL sensitivity in TRAIL-resistant DLD-1/TRAIL and MCF10A cells.

Figure 9

A. Control and DR5 expression plasmid vectors (0.4 μg/ml) were used to transfect DLD-1/TRAIL cells for 24 h, and the cells were then exposed to rTRAIL (5, 10, 25, 50 ng/ml) for 24 h. B. MCF10A cells were transfected with control or DR5 expression plasmid vectors (0.4 μg/ml) for 24 h, and then exposed to rTRAIL (5, 10, 50, 100 ng/ml) for 24 h. The cell viability was estimated at 24 h after the treatment. Data were obtained from 3 independent experiments. The cell viability of the control (0; PBS alone) is indicated as 100%. C and D. Western blot analysis was performed to determine the expression of DR5, activation of caspase-8 and caspase-3 with β-actin used as the internal control.