Figure 4. 5-HT_1DR modulated invasion and migration in colorectal cancer cell.

A & D. Wound-healing assay. Images were taken 0, 24 and 48 hours after wound formation. Data are presented as mean ± SD of triplicate experiments.*P < 0.05, **P < 0.01 ^##P < 0.01 in Fig. 4D. B & C. Cell invasion assay result using Matrigel-coated Transwell. (left, representative pictures of invasion chambers; right, average counts from five random microscopic fields). Data are presented as mean ± SD of triplicate experiments. *P < 0.05 vs. Vector group in Fig. 4A; *P < 0.05, **P < 0.01 in Fig. 4B. D.The cell proliferation of GR127935-treated LoVo cells in different concentration at 24 h, 48 h, 72 h, 96 h as described in Materials. E. Cell proliferation was assayed at 24, 48, 72 and 96 hours after LoVo cells were transfected with Axin1 lentivirus (Axin1^over) or treated with sumatriptan or treated with both together.