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. 2015 Jul 20;6(28):26029–26040. doi: 10.18632/oncotarget.4347

Figure 2. ARW741L is recruited to cis-regulatory elements of AR-target genes and drives a pro-proliferative phenotype in response to bicalutamide.

Figure 2

A. LNCaP-ARW741L cells depleted of either endogenous or ectopic AR were treated with 1 nM DHT or 10 nM bicalutamide for 4 hours prior to chromatin immunoprecipitation (ChIP) analysis using a FLAG antibody to immunoprecipitate FLAG-ARW741L. Receptor recruitment to the PSA enhancer (PSA Enh) and TMPRSS2 promoter was assessed by quantitative PCR. B. LNCaP-ARW741L cells depleted of endogenous receptor and treated with 1 nM DHT or 10 nM bicalutamide for 4 hours were subject to ChIP analysis using a phospho-Serine 5 RNA polymerase II antibody (pSer5 Pol II) and enrichment at PSA and TMPRSS2 genes measured by quantitative PCR. C. LNCaP cells or the LNCaP-ARW741L derivative depleted of endogenous or ectopic AR were grown in the presence of 1 nM DHT or 10 nM bicalutamide for 96 hours prior to SRB staining. Percentage growth is relative to vehicle control for each siRNA. Data is the mean of triplicate experiments ± standard error.