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. 2015 Jul 20;6(28):26052–26064. doi: 10.18632/oncotarget.4482

Figure 2. LIFR and LIF expression in human primary and established CCA cell lines.

Figure 2

By WB, LIFR protein levels were higher although variable in primary (n = 7) and established (n = 3) CCA cell lines compared with control (n = 2) cholangiocytes A. Using ELISA, LIF was found to be secreted by both neoplastic and control cholangiocytes, however with a large variability B. Of the established CCA cell lines, only HuCCT-1 and TFK-1 cells expressed LIFR C. and LIF D., as shown by immunocytochemistry, which were then selected for in vitro experiments (Original magnification: 200x; *p < 0.05 vs. primary controls).